A Novel Role for Phosphatidylinositol 3-Kinase β in Signaling from G Protein-coupled Receptors to Akt

摘要: The protein kinase Akt plays a central role in number of key biological functions including synthesis, glucose homeostasis, and the regulation cell survival or death. mechanism by which tyrosine growth factor receptors stimulate has been recently defined. In contrast, activation other surface is much less understood. For G protein-coupled (GPCRs), conflicting data suggest that these type-specific manner yet to be fully elucidated mechanism. Here, we took advantage availability cells, where activity could not enhanced agonists acting on this large family receptors, such as NIH 3T3 investigate pathway linking GPCRs Akt. We present evidence expression phosphatidylinositol 3-kinase (PI3K) beta necessary sufficient transmit signals from proteins murine fibroblasts PI3Kbeta may represent most likely whereby Akt, vast majority cells do express PI3Kgamma, known protein-sensitive PI3K isoform. Furthermore, available indicates activate distinct utilized it involves phosphorylation-independent betagamma dimers.