Microsatellite markers derived from Quercus mongolica var. crispula (Fagaceae) inner bark expressed sequence tags.
作者： Saneyoshi Ueno , Yuriko Taguchi , Yoshihiko Tsumura
DOI: 10.1266/GGS.83.179
关键词:
摘要: In reforestation programs the genetic composition and diversity of populations that could be used as sources planting material needs to carefully considered maximize chances successful establishment. For such purposes analyses include identification functional genes are required. this study, we constructed a cDNA library from inner bark Quercus mongolica (which is widely distributed in Japan) collected 3385 ESTs. After constructing 2140 unigenes, 274 microsatellites were found within them. The most frequent microsatellite had AG motif (48%) next common was AAG (12%). There no CG repeats unigenes. total, 20 EST-SSR markers developed, polymorphisms which described by using eight individuals over species' distributional range. number alleles per locus (Na) observed heterozygosity (H(o)) ranged 2 12, 0.25 1.00, respectively. Cross-species amplification for 19 loci Q. serrata dentata, with values Na H(o) comparable those mongolica. characterized study should facilitate analysis future studies.
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