Identification of the antiphagocytic trypacidin gene cluster in the human-pathogenic fungus Aspergillus fumigatus

摘要: The opportunistic human pathogen Aspergillus fumigatus produces numerous different natural products. genetic basis for the biosynthesis of a number known metabolites has remained unknown. gene cluster encoding conidia-bound metabolite trypacidin is particular interest because its antiprotozoal activity and possible role in infection process. Here, we show that genes enzymes reside within an orphan A. fumigatus. Genome mining identified tynC as uncharacterized polyketide synthase with high similarity to enzymes, whose products are structurally related including endocrocin fumicycline. Gene deletion resulted complete absence production, which was fully restored when mutant strain complemented wild-type gene. When confronted macrophages, conidia were more frequently phagocytosed than those parental strain. This also found phagocytic amoebae species Dictyostelium discoideum, showed increased phagocytosis ΔtynC conidia. Both macrophages sensitive trypacidin. Therefore, our results suggest conidium-bound could have protective function against phagocytes both environment during