作者: Molly Brewer , J. Taylor Wharton , Jian Wang , Amanda McWatters , Nelly Auersperg
DOI: 10.1016/J.YGYNO.2005.01.051
关键词:
摘要: Abstract Background. Epithelial ovarian cancer has the highest mortality rate among gynecologic cancers. The synthetic retinoid, N-(4-hydroxyphenyl) retinamide (4-HPR), been used in chemoprevention of cancer. However, effectiveness its application for different populations questioned because genetic differences normal, high risk, and women with Objective. To explore similarities 4-HPR effects on epithelial cells which mimic women, normal surface epithelium to represent population immortalized premalignant changes, derived from malignant changes were as vitro models. Methods. Normal cells, incubated intervals increasing concentrations 4-HPR. Growth inhibition, fraction apoptotic expression apoptosis-related genes, including p53, p16, p21, caspase-3, mitochondrial permeability transition measured before after treatment. Results. Treatment produced growth inhibition apoptosis a dose-dependent manner all 3 cell types. strongest activation p53 pathway (NOE) while it caused largest increase MPT suggesting mechanism and/or these lines. 4-HPR, at concentration 10 μM, had maximal effect caspase-3 activity 72 h 48 although modest. Conclusions. showed differential response Although same endpoints induction present may be regulated through pathways. Implications. Clinical trials higher should prove beneficial.