A method for determining states in the course of protein unfolding
作者： Mostafa Rezaei-Tavirani , Sayed-Amir Marashi , Seyed Mohammad Mahdavi
DOI: 10.17877/DE290R-122
关键词:
摘要: A simple model is presented for analyzing a set of spectra obtained from spectrophotometric study protein titration. With this one can determine the states (probable) intermediates in course unfolding. The developed based on abundance native state, intermediate(s) and denatured their contributions to differential absorbance at selected wavelengths. tested two-state unfolding ribonuclease by urea formate buffer, also three-state α-lactalbumin guanidine hydrochloride (GdnHCl) phosphate buffer. It was demonstrated that matched acceptably with model, while starts twostate mechanism (when [GdnHCl]≥1.6M) followed pathway.
tu-dortmund.de 
sci-hub.st 参考文章(9)
Kunihiro Kuwajima, Katsutoshi Nitta, Michio Yoneyama, Shintaro Sugai, Three-state denaturation of α-lactalbumin by guanidine hydrochloride Journal of Molecular Biology. ,vol. 106, pp. 359- 373 ,(1976) , 10.1016/0022-2836(76)90091-7
I. J. Ropson, C. Frieden, Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 7222- 7226 ,(1992) , 10.1073/PNAS.89.15.7222
Dharmaraj Samuel, Thallampuranam Krishnaswamy Suresh Kumar, Thiagarajan Srimathi, Hui-chu Hsieh, Chin Yu, Identification and Characterization of an Equilibrium Intermediate in the Unfolding Pathway of an All β-Barrel Protein Journal of Biological Chemistry. ,vol. 275, pp. 34968- 34975 ,(2000) , 10.1074/JBC.M005147200
C PACE, Measuring and increasing protein stability Trends in Biotechnology. ,vol. 8, pp. 93- 98 ,(1990) , 10.1016/0167-7799(90)90146-O
N.F. Martins, M.M. Santoro, Partially folded intermediates during trypsinogen denaturation. Brazilian Journal of Medical and Biological Research. ,vol. 32, pp. 673- 682 ,(1999) , 10.1590/S0100-879X1999000600002
J.M. Yon, Protein folding: a perspective for biology, medicine and biotechnology Brazilian Journal of Medical and Biological Research. ,vol. 34, pp. 419- 435 ,(2001) , 10.1590/S0100-879X2001000400001
P L Privalou, Thermodynamic Problems of Protein Structure Annual Review of Biophysics and Biophysical Chemistry. ,vol. 18, pp. 47- 69 ,(1989) , 10.1146/ANNUREV.BB.18.060189.000403
Allel Chedad, Herman Van Dael, Kinetics of folding and unfolding of goat α-lactalbumin Proteins: Structure, Function, and Bioinformatics. ,vol. 57, pp. 345- 356 ,(2004) , 10.1002/PROT.20212
Aaron Nieuwsma, Applied Multivariate Statistical Methods ,(2005)