Semliki Forest virus A7(74) transduces hippocampal neurons and glial cells in a temperature-dependent dual manner.

摘要: In central nervous system (CNS) tissue preparations, wild-type Semliki Forest virus (SFV) mainly infects neurons, and in vivo it causes lethal encephalitis neonatal adult rodents. The SFV strain A7(74), by contrast, is avirulent rodents, triggering only limited CNS infection. To examine A7(74) infection hippocampal tissue, the authors constructed a replicon, termed SFV(A774nsP)-GFP, expressing green fluorescent protein. results were compared to replication-proficient recombinant encoding GFP, named VA7-EGFP. As nonstructural gene mutations can confer temperature sensitivity, also tested whether was temperature-dependent. Indeed, at 31°C both viral recombinants transduced significantly more baby hamster kidney cells than 37°C. When rat slices dissociated incubated 37°C, SFV(A774nsP)-GFP glial but virtually no neurons—the opposite of conventional SFV. For VA7-EGFP preferred GFP-positive non-neuronal cells. At 31°C, however, phenotype found, with 33% 94% being neurons for cells, respectively, slices. Immunochemical electrophysiological analyses confirmed that 37°C all astrocytes, while they contained neurons. These show addition developmental age, determines which cell type becomes infected A7(74). Our data suggest animals because does not readily replicate mature body temperature, whereas still so lower temperatures.