Recombinant human serum amyloid P component from Pichia pastoris: production and characterization
作者： Susanne Boysen , Berit Fogh-Schultz , Inger Andersen , Peter Højrup , Jens Jørgen Lønsmann Iversen
DOI: 10.1016/J.PEP.2004.01.011
关键词:
摘要: Abstract Human serum amyloid P component (SAP) was expressed in the methylotrophic yeast Pichia pastoris . SAP cDNA placed under control of regulatory sequences derived from alcohol oxidase gene (AOX1), and its protein product secreted using Saccharomyces cerevisiae α-mating factor signal sequence. Recombinant (r-SAP) produced a bioreactor with computer controlled fed-batch mode purified by use C-terminal histidine tag. The yield r-SAP 3–4 mg 1 L supernatant 5–6 mg cell paste, indicating that majority not secreted. Treatment paste EDTA increased further about 30%. N-terminal showed non-complete cleavage Purified r-SAP, analyzed native conditions, shown to be decamer, like human (h-SAP), monomers 27 kDa. Each monomer had one N-glycosylation site, positioned at same site as for h-SAP. bound antibodies against Furthermore, ds DNA influenza A virus subunits Ca 2+ -dependent manner inhibited hemagglutination. These results indicate P. has biological activity
core.ac.uk
elsevier.com
sci-hub.se 参考文章(29)
M. E. Gottesman, S. Ito, H. Kawano, V. Episkopou, K. Horie, T. Ishihara, F. Costantini, S. Maeda, Y. Okada, S.-K. Lim, K. Shimada, S. Togashi, T. Kinoshita, S. Nakano, Serum amyloid P component enhances induction of murine amyloidosis. Laboratory Investigation. ,vol. 77, pp. 525- 531 ,(1997)
Inge Juul Sørensen, Complexes of serum amyloid P component and DNA in serum from healthy individuals and systemic lupus erythematosus patients. Journal of Clinical Immunology. ,vol. 20, pp. 408- 415 ,(2000) , 10.1023/A:1026478914129
Ellen Holm Nielsen, Mads Nybo, Sven-Erik Svehag, Electron microscopy of prefibrillar structures and amyloid fibrils. Methods in Enzymology. ,vol. 309, pp. 491- 496 ,(1999) , 10.1016/S0076-6879(99)09033-3
M.C.M. Bickerstaff, M. Botto, W.L. Hutchinson, J. Herbert, G.A. Tennent, A. Bybee, D.A. Mitchell, H.T. Cook, P.J.G. Butler, M.J. Walport, M.B. Pepys, Serum amyloid P component controls chromatin degradation and prevents antinuclear autoimmunity Nature Medicine. ,vol. 5, pp. 694- 697 ,(1999) , 10.1038/9544
E C Mantzouranis, S B Dowton, A S Whitehead, M D Edge, G A Bruns, H R Colten, Human serum amyloid P component. cDNA isolation, complete sequence of pre-serum amyloid P component, and localization of the gene to chromosome 1. Journal of Biological Chemistry. ,vol. 260, pp. 7752- 7756 ,(1985) , 10.1016/S0021-9258(17)39671-0
J. J. L. Iversen, J. K. Thomsen, R. P. Cox, On-line growth measurements in bioreactors by titrating metabolic proton exchange Applied Microbiology and Biotechnology. ,vol. 42, pp. 256- 262 ,(1994) , 10.1007/BF00902726
Juul Sørensen, O. Andersen, E. Holm Nielsen, S.-E. Svehag, Multiple isoforms of the human pentraxin serum amyloid P component International Archives of Allergy and Immunology. ,vol. 106, pp. 25- 31 ,(1995) , 10.1159/000236886
R. A. BRIERLEY, C. BUSSINEAU, R. KOSSON, A. MELTON, R. S. SIEGEL, Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Annals of the New York Academy of Sciences. ,vol. 589, pp. 350- 362 ,(1990) , 10.1111/J.1749-6632.1990.TB24257.X
M.B. Pepys, P.J.G. Butler, Serum amyloid P component is the major calcium-dependent specific DNA binding protein of the serum. Biochemical and Biophysical Research Communications. ,vol. 148, pp. 308- 313 ,(1987) , 10.1016/0006-291X(87)91111-9
J. M. Cregg, J. F. Tschopp, C. Stillman, R. Siegel, M. Akong, W. S. Craig, R. G. Buckholz, K. R. Madden, P. A. Kellaris, G. R. Davis, B. L. Smiley, J. Cruze, R. Torregrossa, G. Velicȩlebi, G. P. Thill, High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris Nature Biotechnology. ,vol. 5, pp. 479- 485 ,(1987) , 10.1038/NBT0587-479