In vitro gene delivery mediated by lactosylated dendrimer (generation 3, G3)/α-cyclodextrin conjugates into hepatocytes
作者： Keiichi Motoyama , Yoshimasa Mori , Shogo Yamashita , Yuya Hayashi , Hirofumi Jono
DOI: 10.1007/S10847-010-9842-0
关键词:
摘要: The purpose of this study is to evaluate in vitro gene delivery efficiency polyamidoamine (PAMAM) starburst dendrimer (generation 3, G3) conjugates with α-cyclodextrin (α-CDE (G3)) bearing lactose (Lac-α-CDE) various degrees substitution the moiety (DSL) as a novel hepatocyte-selective carrier. Lac-α-CDE (G3, DSL 1.2) was found have much higher transfer activity than α-CDE (G3), (G2, 2.6) and Lac-α-CDEs 2.6, 4.1 6.1) HepG2 cells, which are dependent on expression cell-surface asialoglycoprotein receptor (ASGP-R). provided negligible cytotoxicity up charge ratio 100 (carrier/pDNA) cells. These results suggest potential use non-viral vector for toward hepatocytes.
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