2-Aminopurine hairpin probes for the detection of ultraviolet-induced DNA damage.
作者： Amira F. El-Yazbi , Glen R. Loppnow
DOI: 10.1016/J.ACA.2012.03.021
关键词:
摘要: Abstract Nucleic acid exposure to radiation and chemical insults leads damage disease. Thus, detection understanding DNA is important for elucidating molecular mechanisms of However, current methods are either time-consuming, destroy the sample, or too specific be used generic damage. In this paper, we develop a fluorescence sensor 2-aminopurine (2AP), fluorescent analogue adenine, incorporated in loop hairpin probe quantification ultraviolet (UV) C-induced nucleic Our results show that selectivity 2AP UV-induced comparable beacon (MB) probes The calibration curve shows good linearity (R2 = 0.98) with limit 17.2 nM. This simple, fast economic DNA.
elsevier.com
sci-hub.se 参考文章(35)
Colleen C. Trevithick-Sutton, Larisa Mikelsons, Vasilisa Filippenko, J. C. Scaiano, Effect of UVC-induced damage to DNA on the intercalation of thiazole orange: a convenient reporter for DNA damage. Photochemistry and Photobiology. ,vol. 83, pp. 556- 562 ,(2007) , 10.1562/2006-07-21-RA-976
D C Ward, E Reich, L Stryer, Fluorescence studies of nucleotides and polynucleotides. I. Formycin, 2-aminopurine riboside, 2,6-diaminopurine riboside, and their derivatives. Journal of Biological Chemistry. ,vol. 244, pp. 1228- 1237 ,(1972) , 10.1016/S0021-9258(18)91833-8
Arvind Misra, P. Kumar, K.C. Gupta, Synthesis of hairpin probe using deoxyguanosine as a quencher: Fluorescence and hybridization studies. Analytical Biochemistry. ,vol. 364, pp. 86- 88 ,(2007) , 10.1016/J.AB.2007.02.003
Arvind Misra, Mohammad Shahid, Immobilization of self-quenched DNA hairpin probe with a heterobifunctional reagent on a glass surface for sensitive detection of oligonucleotides Bioorganic & Medicinal Chemistry. ,vol. 17, pp. 5826- 5833 ,(2009) , 10.1016/J.BMC.2009.07.015
Laurent Marrot, Jean-Roch Meunier, Skin DNA photodamage and its biological consequences Journal of the American Academy of Dermatology. ,vol. 58, pp. S139- S148 ,(2008) , 10.1016/J.JAAD.2007.12.007
Edward L. Rachofsky, Roman Osman, J. B. Alexander Ross, Probing structure and dynamics of DNA with 2-aminopurine: effects of local environment on fluorescence. Biochemistry. ,vol. 40, pp. 946- 956 ,(2001) , 10.1021/BI001664O
Usharani Subuddhi, Matthew Hogg, Linda J. Reha-Krantz, Use of 2-Aminopurine Fluorescence To Study the Role of the β Hairpin in the Proofreading Pathway Catalyzed by the Phage T4 and RB69 DNA Polymerases Biochemistry. ,vol. 47, pp. 6130- 6137 ,(2008) , 10.1021/BI800211F
Jean-Luc Ravanat, Thierry Douki, Jean Cadet, Direct and indirect effects of UV radiation on DNA and its components. Journal of Photochemistry and Photobiology B-biology. ,vol. 63, pp. 88- 102 ,(2001) , 10.1016/S1011-1344(01)00206-8
Christopher R. Guest, Remo A. Hochstrasser, Lawrence C. Sowers, David P. Millar, Dynamics of mismatched base pairs in DNA. Biochemistry. ,vol. 30, pp. 3271- 3279 ,(1991) , 10.1021/BI00227A015
Joseph Wang, Gustavo Rivas, Mehmet Ozsoz, Douglas H. Grant, Xiaohua Cai, Concepción Parrado, Microfabricated electrochemical sensor for the detection of radiation-induced DNA damage. Analytical Chemistry. ,vol. 69, pp. 1457- 1460 ,(1997) , 10.1021/AC961000D