Digital pcr systems and methods using digital microfluidics
作者： Stanford Kwang
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摘要: Systems and methods are described for performing digital PCR using microfluidics configured precise movement of picoliter to nanoliter sized partitions which can be used partition generation, through a temperature gradient nucleic acid melting, signal detection all within single consumable device.
google.de 参考文章(29)
Vamsee K. Pamula, Philip Y. Paik, Michael G. Pollack, Richard B. Fair, Hong Ren, Methods and apparatus for manipulating droplets by electrowetting-based techniques ,(2003)
Benoît Limoges, Damien Marchal, Thibaut Defever, Method for electrochemically identifying target nucleotide sequences ,(2009)
R. Erik Holmlin, Eric D. A. Stemp, Jacqueline K. Barton, Os(phen)2dppz2+ in Photoinduced DNA-Mediated Electron Transfer Reactions Journal of the American Chemical Society. ,vol. 118, pp. 5236- 5244 ,(1996) , 10.1021/JA953941Y
Feng Shen, Bing Sun, Jason E. Kreutz, Elena K. Davydova, Wenbin Du, Poluru L. Reddy, Loren J. Joseph, Rustem F. Ismagilov, Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and Hepatitis C viral load Journal of the American Chemical Society. ,vol. 133, pp. 17705- 17712 ,(2011) , 10.1021/JA2060116
Shahida N. Syed, Holger Schulze, Daniel Macdonald, Jason Crain, Andrew R. Mount, Till T. Bachmann, Cyclic denaturation and renaturation of double-stranded DNA by redox-state switching of DNA intercalators. Journal of the American Chemical Society. ,vol. 135, pp. 5399- 5407 ,(2013) , 10.1021/JA311873T
Rodrigo Staggemeier, Diogo André Pilger, Fernando Rosado Spilki, Vlademir Vicente Cantarelli, Multiplex SYBR® green-real time PCR (qPCR) assay for the detection and differentiation of Bartonella henselae and Bartonella clarridgeiae in cats. Revista Do Instituto De Medicina Tropical De Sao Paulo. ,vol. 56, pp. 93- 95 ,(2014) , 10.1590/S0036-46652014000200001
M. R. Arkin, E. D. A. Stemp, R. E. Holmlin, J. K. Barton, A. Hormann, E. J. C. Olson, P. F. Barbara, Rates of DNA-Mediated Electron Transfer Between Metallointercalators Science. ,vol. 273, pp. 475- 480 ,(1996) , 10.1126/SCIENCE.273.5274.475
Ádám Horváth, Zoltán Pető, Edit Urbán, Csaba Vágvölgyi, Ferenc Somogyvári, None, A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria BMC Microbiology. ,vol. 13, pp. 300- 300 ,(2013) , 10.1186/1471-2180-13-300
Shinobu SATO, Shigeori TAKENAKA, Electrochemical DNA Detection Using Supramolecular Interactions Analytical Sciences. ,vol. 28, pp. 643- 649 ,(2012) , 10.2116/ANALSCI.28.643
Pornpat Athamanolap, Vishwa Parekh, Stephanie I. Fraley, Vatsal Agarwal, Dong J. Shin, Michael A. Jacobs, Tza-Huei Wang, Samuel Yang, Trainable High Resolution Melt Curve Machine Learning Classifier for Large-Scale Reliable Genotyping of Sequence Variants PLoS ONE. ,vol. 9, pp. e109094- ,(2014) , 10.1371/JOURNAL.PONE.0109094