Methods and devices for digital PCR
作者： Tara Dalton , Mark B. Davies
DOI:
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摘要: The invention provides methods of conducting a nucleic acid reaction, including for performing digital PCR using “droplet-in-oil” technology. In the methods, starting sampled is segmented at least partially into set sample droplets each containing on average about one or fewer copies target acid. are passed in continuous flow immiscible carrier fluid through channel that passes thermal cycler, whereby amplified. implementation, 350 nl and number positively amplified counted near-saturation point.
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