Transcripts within rod photoreceptors of the Zebrafish retina.

摘要: The purpose of this study was to identify transcripts retinal rod photoreceptors the zebrafish. zebrafish is an important animal model for vision science due rapid and tractable development, persistent neurogenesis rods throughout lifespan, capacity functional regeneration. Zebrafish rods, non-rod cells xops:eGFP transgenic line, were separated by cell dissociation fluorescence-activated sorting (FACS), followed RNA-seq. At a false discovery rate < 0.01, 597 upregulated (“enriched”) in vs. other cells, 1032 downregulated (“depleted”). Thirteen thousand three hundred twenty four total detected including many not previously known be expressed rods. Forty five validated qPCR FACS-sorted cells. Transcripts enriched from adult retinas also larval juvenile retinas, sorted subjected neurotoxic lesion allowed regenerate. Many wildtype those degeneration. We report generation validation RNA-seq dataset describing transcriptome zebrafish, which now available as resource further studies photoreceptor biology comparative transcriptomics.