Does the autoantibody immunodominant region on thyroid peroxidase include amino acid residues 742-771?

摘要: Identification of the thyroid peroxidase (TPO) amino acid residues that comprise autoantibody immunodominant region is an important goal has proven difficult because conformational nature epitopes involved. Recent data suggest been located. Thus, by recognition tryptic fragments native TPO, as well portions TPO expressed cell-free translates, appears to include 742–771, near C terminus ectodomain. To evaluate this deduction, we translates full ectodomain, truncated after 741 and 771. The epitopic integrity these molecules was first confirmed immunoprecipitation patient sera containing autoantibodies. However, could involve a minority autoantibodies with individual sera, not fulfilling strict criteria for immunodominance. In orde...