Cobalt(II) complexes of terpyridine bases as photochemotherapeutic agents showing cellular uptake and photocytotoxicity in visible light

摘要: Cobalt(II) complexes of terpyridine bases [Co(L)2](ClO4)2 (1–3), where L is 4′-phenyl-2,2′:6′,2′′-terpyridine (ph-tpy in 1), 4′-(9-anthracenyl)-2,2′:6′,2′′-terpyridine (an-tpy 2) and 4′-(1- pyrenyl)-2,2′:6′,2′′-terpyridine (py-tpy 3), are prepared their photo-induced DNA protein cleavage activity photocytotoxic property HeLa cells studied. The 1 : 2 electrolytic three-electron paramagnetic show a visible band near 550 nm DMF–Tris-HCl buffer. 1–3 emission spectral bands at 355, 421 454 nm, respectively, when excited 287, 368 335 nm. quantum yield values for DMF–H2O (2 : 1 v/v) 0.025, 0.060 0.28, respectively. redox active DMF–0.1 M TBAP. Co(III)–Co(II) Co(II)–Co(I) couples appear as quasi-reversible cyclic voltammetric responses 0.2 −0.7 V vs. SCE, Complexes 2 3 avid binders to calf thymus giving Kb value ∼106 M−1. chemical nuclease activity. exhibit oxidative pUC19 UV-A light. photocleavage reaction 365 shows formation singlet oxygen hydroxyl radical species, while only evidenced non-specific bovine serum albumin an-tpy py-tpy significant photocytotoxicity cervical cancer on exposure light IC50 24.2 7.6 μM, Live cell imaging study accumulation the cytosol cells.