Analytical Procedures for the Sequential Extraction of 14C‐Labeled Constituents from Leaves, Bark and Wood of Cottonwood Plants

摘要: A procedure for the extraction, separation, and measurement of photosynthetically fixed 14C in up to 8 chemical fractions (CHCl), amino acids, organic sugars, protein, starch, hemicellulose, residue) from small samples (1 100 mg) cottonwood (Populus deltoides Bartr.) leaf material is described. The different are extracted a sequence chemical, ion exchange, enzymatic steps. 14C-activity these major then determined with liquid scintillation spectrometry. These (e.g., acids) can be further separated into their individual components by standard thin-layer or gas- chromatographic methods quantitative analysis if specific activities desired. advantage that many sequentially good reproducibility amount plant without transfer original homogenizer centrifuge tube.