Pseudomonas Aeruginosa-Candida Albicans Interactions From Ecological and Molecular Perspectives

摘要: Pseudomonas aeruginosa and Candida albicans have shown antagonistic relationships, both in vivo vitro. The interaction between P. C. is one of the many prokaryotic-eukaryotic interactions existing nature needs more research due to their complex pathogenicity humans. In this work, we studied growth dynamics a mixed population Candida, receptor ligand specificities, from an ecological molecular point view. Initially, growth, viability, morphogenesis were presence conditioned medium using two strains, namely CAF2 tup1 mutant. killing effect was pronounced on hyphal form Candida. However, inhibited by irrespective its morphological form. had no rate Nevertheless, it completely germination attachment different strains both, with cells harvested at stages growth. percent varied age culture. A lecB mutant showed two–fold reduction compared wild type PAK strain. Carbohydrate inhibition studies confirmed that LecB not directly involved mechanism, but indirectly involves through regulating expression other proteins required for attachment. genomic DNA library PAO1 screened clones acquired ability attach albicans. clone isolated small increase subjected genetic analysis. It contained nucleotides 458565 475917 genome including some genes Pil-Chp gene cluster. Seven transposon mutants represent mutations ChpA,B,C,D,E operon three ORFs selected, tested. All seven indicating non specific effect, which could be attributed vitro manipulation bacteria. We conclude multi-factorial. INDEX WORDS: aeruginosa, albicans, Attachment, Morphogenesis, Adhesins, LecB, inhibition, Primary attachment, PSEUDOMONAS AERUGINOSA CANDIDA ALBICANS INTERACTIONS FROM ECOLOGICAL AND MOLECULAR PERSPECTIVES