Regulation of radiation-induced apoptosis in oncogene-transfected fibroblasts: influence of H-ras on the G2 delay.

摘要: Primary fibroblasts, after serum withdrawal or irradiation, do not undergo apoptosis. Myc-transfected in contrast, apoptosis upon and irradiation. We have studied the relationship of induction to effects on G2 phase cell cycle a series rat embryo cells transformed by rasH plus myc immortalized alone. In this system, while presence had little effect extent withdrawal, greatly suppressed apoptotic response myc-transfected X-rays. The into which been introduced showed profound arrest associated with suppression cyclin B1 mRNA expression. alone minimal delay irradiation no hypothesize that rasH, influencing X-rays, exerts an anti-apoptotic effect. support hypothesis; we found treatment caffeine, agent relieves resulted increased irradiated cells, but control cells.