Target irradiation induced bystander effects between stem-like and non stem-like cancer cells

摘要: Tumors are heterogeneous in nature and consist of multiple cell types. Among them, cancer stem-like cells (CSCs) suggested to be the principal cause tumor metastasis, resistance recurrence. Therefore, understanding behavior CSCs direct indirect irradiations is crucial for clinical radiotherapy. Here, their counterpart non (NSCCs) human HT1080 fibrosarcoma line were sorted labeled, then two subtypes mixed together chosen separately irradiated via a proton microbeam. The radiation-induced bystander effect (RIBE) between NSCCs was measured by imaging 53BP1 foci, widely used indicator DNA double strand break (DSB). found less active than both generation response signals. Moreover, nitric oxide (NO) scavenger c-PTIO can effectively alleviate but not CSCs, indicating difference NO signal response. To our knowledge, this first report shedding light on RIBE NSCCs, which might contribute further out-of-field