Genetic and Expression Analysis of HER-2 and EGFR Genes in Salivary Duct Carcinoma: Empirical and Therapeutic Significance

摘要: Purpose: Salivary duct carcinoma overexpresses epidermal growth factor receptor (EGFR) and HER-2, although the underlying mechanisms remain undefined. Because of potential utilization these markers as treatment targets, we evaluated protein gene status by several techniques to determine complementary value. Experimental Design: A tissue microarray 66 salivary carcinomas was used for immunohistochemical analysis HER-2 EGFR expression (semiquantitatively into a three-tiered system), fluorescence in situ hybridization copy number, chromosomes 7 17 ploidy status. Sequencing exons 18, 19, 21 mutations carried out. Result: For , 46 (69.7%) tumors showed some form (17 at 3+, 2+, 12 1+) but none amplification. Five (9.4%) 53 exon 18 ( n = 3) 19 2). Polysomy chromosome (average >2.5 copies/cell) detected 15 (25.0%) 60 (6 5 2 1+, 0+ expression) correlated with poor 3-year survival P 0.015). (25.8%) expressed (10 3 4 1+). Eight amplification 1 expression). Chromosome polysomy found 8 (15.7%) 51 tumors; two had (3+, Conclusion: Our study shows that ) harbor subset may guide therapy, b pursue an aggressive clinical course cases high expression, c be selected targeted therapy. Clin Cancer Res; 16(8); 2266–74. ©2010 AACR.