Mouse bronchiolar cell carcinogenesis. Histologic characterization and expression of Clara cell antigen in lesions induced by N-nitrosobis-(2-chloroethyl) ureas.

摘要: Female Swiss mice (Cr:NIH(S)) developed bronchiolar cell hyperplasia, dysplasia, metaplasia, and various morphologic types of tumors after topical (skin) application N-nitroso-methyl-bis-chloroethylurea (NMBCU) or N-nitroso-tris-chloroethylurea (NTCU). These compounds are the first found to induce systemically in high incidence. Twice a week, with 3-day interval, 25-microliter drop 0.04 mol/l (molar) NMBCU NTCU acetone was applied shaved interscapular integument for maximum 35 40 weeks. The earliest lung neoplasms were seen that died 23 weeks treatment affected 11 19 14 treatment. Tumor growth pattern nodular neoplastic tissue frequently disseminated throughout parenchyma, starting from multicentric peribronchiolar foci. most common tumor squamous carcinomas adenosquamous carcinomas, followed by adenocarcinomas without secretory cells, single ciliated-cell tumor. Histochemical immunohistochemical studies carried out on paraffin-embedded lungs using avidin-biotin immunoperoxidase complex procedure antisera against keratin, Clara antigen, surfactant apoprotein, neuron-specific enolase, bombesin, chromogranin A. In several both groups, hyperplasias composed cells expressing antigen. No alveolar type II neuroendocrine markers. It appeared particular had migrated terminal bronchioles invaded walls extend into parenchyma. Squamous metaplasia keratin expression within airways associated glandular tumors, especially at periphery. A unique type, large eosinophilic globules crystals, lining forming hyperplastic lesions. N-nitroso-methyl-bis-chloroethylurea- NTCU-induced mouse alterations could be an interesting new model study mechanisms differentiation formation.