Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages
作者： Dominik Spensberger , Ekaterini Kotsopoulou , Rita Ferreira , Cyril Broccardo , Linda M Scott
DOI: 10.1016/J.EXPHEM.2012.02.006
关键词:
摘要: The stem cell leukemia (Scl)/Tal1 gene is essential for normal blood and endothelial development, expressed in hematopoietic cells (HSCs), progenitors, erythroid, megakaryocytic, mast cells. Scl +19 enhancer active HSCs progenitor cells, megakaryocytes, but not mature erythroid Here we demonstrate that in vivo deletion of the (SclΔ19/Δ19) results viable mice with expression lineages. By contrast, reduced stem/progenitor compartment flow cytometry analysis revealed HSC megakaryocyte-erythroid populations are enlarged SclΔ19/Δ19 mice. increase numbers contributed to enhanced expansion bone marrow transplantation assays, did affect multilineage repopulation or stress responses. These affirm plays a key role development necessary Moreover, histone marks across locus were significantly fetal liver without major changes steady-state messenger RNA levels, suggesting post-transcriptional compensation loss regulatory element, result might be widely relevant given frequent observation mild phenotypes after elements.
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