Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis

摘要: An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production food additives and nutraceuticals industries. In this study, xylanase of pretreated hemicellulosic kenaf (Hibiscus cannabinus) substantial was demonstrated. Experiments were performed primarily to investigate the effect several critical reaction conditions towards enzymatic pre-treated kenaf. The effects various ranges (substrate loading, pH, temperature time) systematically thoroughly studied. study conversion using single enzyme proven effectively yield up 59% after 48 h incubation 3% (w/v) substrate loading 400 U at 50°C in pH 4.0 buffer systems. use on key parameter understanding action main degrading hydrolysing backbone Enzyme feeding strategy also conducted results revealed that batch yielded highest compared other fed strategy. It concluded each gave significant impact optimum resulted high product end reaction. With discovery importance involved hydrolysis, highlights need comprehensive investigation mixture enzymes achieving complete with higher yield.