Organization of the transcription factor binding sites in the kappa Ig intron enhancer. Effects of position, orientation, and spacing.

摘要: The kappa Ig intron enhancer is comprised of multiple sequence motifs known to bind trans-acting factors that activate gene expression. A species comparison reveals a high level conservation the organization transcription factor binding sites within enhancer. importance conserved was examined by using topologic mutations disrupt position, orientation, and spacing individual effects these changes were monitored their on reporter activity at two distinct stages B cell development. Previously, mutational analysis indicated E2 be most crucial for function. We have demonstrated dependent position Intron function is, however, independent site orientation flexible in requirements among sites.