Influence of fixation and immunohistological technique on accuracy, precision and inter-observer reproducibility of plasma cell counting.

作者: Bodeutsch C , Vooijs Gp , Kater L , Ebben Gp , van Houwelingen Jc

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摘要: Recently two highly sensitive and specific diagnostic criteria for Sjogren's syndrome based on percentages of IgA-, IgG-, IgM-containing plasma cells measured in immunohistologically stained labial salivary gland tissue have been described. The reliability such a criterion is dependent the accuracy, precision inter-observer reproducibility cell counting. present study evaluates effect fixation immunohistological procedures aforementioned factors. Immunoglobulin (Ig)-containing sections lamellated submandibular tissue, alternately fixed 4% buffered formol solution or formol-sublimate with an indirect immunoperoxidase unlabelled peroxidase anti-peroxidase (PAP) method respectively, were enumerated by three independent observers. Relative numbers Ig-containing appeared to be less systematic errors due procedure than absolute cells. best counts was obtained from sublimate-fixed specimens according PAP procedure.

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