Biomimetic hydrogels gate transport of calcium ions across cell culture inserts

摘要: Control of the in vitro spatiotemporal availability calcium ions is one means by which microenvironments hematopoietic stem cells grown culture may be reproduced. The effects cross-linking density on diffusivity through cell compatible poly(2-hydroxyethyl methacrylate) [poly(HEMA)]-based bioactive hydrogels possessing 1.0 mol% 2-methacryloyloxyethyl phosphorylcholine (MPC), 5 N,N-(dimethylamino)ethylmethacrylate (DMAEMA) and ca. 17 n-butyl acrylate (n-BA) have been investigated to determine if varying cross-link a viable approach controlling transport across hydrogel membranes. Cross-linking was varied changing composition cross-linker, tetraethyleneglycol diacrylate (TEGDA). membranes were formed sandwich casting onto external surface track-etched polycarbonate (T = 10 μm, φ 0.4 μm pores) inserts, polymerized place UV light irradiation immersed buffered (0.025 HEPES, pH 7.4) 0.10 M chloride solution. membrane monitored using ion selective electrode set within insert. Degree hydration (21.6 ± 1.0%) void fraction found constant all densities. Diffusion coefficients, determined time-lag analysis, shown strongly dependent exponentially decrease with increasing density. Compared that buffer (2.0–2.5 × 10−6 cm2/s), diffusion coefficients ranged from 1.40 cm2/s 1.80 10−7 tortuosity values 1.7 10.0 for 1 12 TEGDA cross-linked respectively. Changes arising variations primary modality novel n-BA containing poly(HEMA)-based hydrogels.