A dual-channel homogeneous aptasensor combining colorimetric with electrochemical strategy for thrombin.
作者： Yaxing Zhang , Jianfei Xia , Feifei Zhang , Zonghua Wang , Qingyun Liu
DOI: 10.1016/J.BIOS.2018.08.031
关键词:
摘要: Abstract In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in single analytical system signal readout. sensing strategy, the G-quadruplex sequence released incorporated with hemin to form DNAzyme naked-eye detection. Meanwhile, hydroxyapatite nanoparticle probe magnetic nanoparticles construct sandwich-type structure generating current when present solution. By introducing two kinds of reporter probes transducers, sensor produced different improve accuracy diversity. results revealed that achieved quantatitive determination low limit detection (0.40 fM) wide range (0.1 fM 1 nM), which offer promise rapid accurate biomolecule.
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