The interaction of proteins with hydroxyapatite: I. Role of protein charge and structure☆
作者： Marina J. Gorbunoff
DOI: 10.1016/0003-2697(84)90239-2
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摘要: The criteria for elution of proteins from hydroxyapatite columns were examined as a function (1) protein isoelectric point (22 with points between 3.5 and 11.0); (2) ionic nature eluant (Na salts PO4, F-, Cl-, SCN-, ClO-4, CaCl2); (3) structural differences related proteins. It was found that can be classified into three groups: basic proteins, which elute at similar, moderate molarities low (less than 0.003 M) Ca2+; acidic about equal PO4 but do not Ca2+ usually Cl-; neutral show strong anion specificity, or SCN-. Furthermore, individual specific polar groups are in general crucial to binding desorption, variations structure, other major loosening, influence strongly the pattern protein-hydroxyapatite interaction.
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sci-hub.se 参考文章(38)
H.T. Wright, J. Kraut, P.E. Wilcox, Comparison of the π-, δ- and γ-chymotrypsin family with α-chymotrypsin Journal of Molecular Biology. ,vol. 37, pp. 363- 366 ,(1968) , 10.1016/0022-2836(68)90275-1
Eugene. Fredericq, H.F. Deutsch, Studies on ovomucoid. Journal of Biological Chemistry. ,vol. 181, pp. 499- 510 ,(1949) , 10.1016/S0021-9258(18)56570-4
Emil L. Smith, J.R. Kimmel, Douglas M. Brown, E. O.P. Thompson, Isolation and properties of a crystalline mercury derivative of a lysozyme from papaya latex. Journal of Biological Chemistry. ,vol. 215, pp. 67- 89 ,(1955) , 10.1016/S0021-9258(18)66018-1
Jean-François Pechère, Hans Neurath, Studies on the autocatalytic activation of trypsinogen. Journal of Biological Chemistry. ,vol. 229, pp. 389- 407 ,(1957) , 10.1016/S0021-9258(18)70625-X
P.B. Sigler, D.M. Blow, B.W. Matthews, R. Henderson, Structure of Crystalline α-Chymotrypsin II. A Preliminary Report Including a Hypothesis for the Activation Mechanism Journal of Molecular Biology. ,vol. 35, pp. 143- 164 ,(1968) , 10.1016/S0022-2836(68)80043-9
Pablo Valenzuela, Myron L. Bender, Preparation and kinetic properties of a new form of chymotrypsin which is active at alkaline pH: α1 chymotrypsin Journal of Biological Chemistry. ,vol. 248, pp. 4909- 4914 ,(1973) , 10.1016/S0021-9258(19)43650-8
T Y Lin, D E Koshland, Carboxyl group modification and the activity of lysozyme. Journal of Biological Chemistry. ,vol. 244, pp. 505- 508 ,(1969) , 10.1016/S0021-9258(18)94459-5
Marina J. Gorbunoff, The interaction of proteins with hydroxyapatite: II. Role of acidic and basic groups Analytical Biochemistry. ,vol. 136, pp. 433- 439 ,(1984) , 10.1016/0003-2697(84)90240-9
D.G. Hoare, D.E. Koshland, A Method for the Quantitative Modification and Estimation of Carboxylic Acid Groups in Proteins Journal of Biological Chemistry. ,vol. 242, pp. 2447- 2453 ,(1967) , 10.1016/S0021-9258(18)95981-8
Hannah L. Oppenheimer, Bernard Labouesse, George P. Hess, Implication of an Ionizing Group in the Control of Conformation and Activity of Chymotrypsin Journal of Biological Chemistry. ,vol. 241, pp. 2720- 2730 ,(1966) , 10.1016/S0021-9258(18)96598-1