CmWRKY1 Enhances the Dehydration Tolerance of Chrysanthemum through the Regulation of ABA-Associated Genes

作者: Qingqing Fan , Aiping Song , Jiafu Jiang , Ting Zhang , Hainan Sun

DOI: 10.1371/JOURNAL.PONE.0150572

关键词:

摘要: WRKY transcription factors serve as antagonistic or synergistic regulators in a variety of abiotic stress responses plants. Here, we show that CmWRKY1, member the group IIb family isolated from Chrysanthemum morifolium, exhibits no transcriptional activation yeast cells. The subcellular localization examination showed CmWRKY1 localizes to nucleus vivo. Furthermore, CmWRKY1-overexpressing transgenic lines exhibit enhanced dehydration tolerance response polyethylene glycol (PEG) treatment compared with wild-type We further confirmed plants suppressed expression levels genes negatively regulated by ABA, such PP2C, ABI1 and ABI2, activated positively PYL2, SnRK2.2, ABF4, MYB2, RAB18, DREB1A. Taken together, our results indicate plays an important role drought chrysanthemum through ABA-mediated pathway.

参考文章(60)
Xinqi Fan, Qi Guo, Peng Xu, YuanYong Gong, Hongmei Shu, Yang Yang, Wanchao Ni, Xianggui Zhang, Xinlian Shen, Transcriptome-Wide Identification of Salt-Responsive Members of the WRKY Gene Family in Gossypium aridum PLOS ONE. ,vol. 10, pp. e0126148- ,(2015) , 10.1371/JOURNAL.PONE.0126148
Peiling Li, Aiping Song, Chunyan Gao, Jiafu Jiang, Sumei Chen, Weimin Fang, Fei Zhang, Fadi Chen, The over-expression of a chrysanthemum WRKY transcription factor enhances aphid resistance. Plant Physiology and Biochemistry. ,vol. 95, pp. 26- 34 ,(2015) , 10.1016/J.PLAPHY.2015.07.002
Deena L. Rushton, Prateek Tripathi, Roel C. Rabara, Jun Lin, Patricia Ringler, Ashley K. Boken, Tanner J. Langum, Lucas Smidt, Darius D. Boomsma, Nicholas J. Emme, Xianfeng Chen, John J. Finer, Qingxi J. Shen, Paul J. Rushton, WRKY transcription factors: key components in abscisic acid signalling Plant Biotechnology Journal. ,vol. 10, pp. 2- 11 ,(2012) , 10.1111/J.1467-7652.2011.00634.X
Peiling Li, Aiping Song, Chunyan Gao, Linxiao Wang, Yinjie Wang, Jing Sun, Jiafu Jiang, Fadi Chen, Sumei Chen, Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants Plant Cell Reports. ,vol. 34, pp. 1365- 1378 ,(2015) , 10.1007/S00299-015-1793-X
Tobias Doerks, Richard R Copley, Jörg Schultz, Chris P Ponting, Peer Bork, Systematic identification of novel protein domain families associated with nuclear functions. Genome Research. ,vol. 12, pp. 47- 56 ,(2002) , 10.1101/GR.203201
Minjie Cao, Xue Liu, Yan Zhang, Xiaoqian Xue, X Edward Zhou, Karsten Melcher, Pan Gao, Fuxing Wang, Liang Zeng, Yang Zhao, Yang Zhao, Pan Deng, Dafang Zhong, Jian-Kang Zhu, H Eric Xu, Yong Xu, An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants. Cell Research. ,vol. 23, pp. 1043- 1054 ,(2013) , 10.1038/CR.2013.95
Xiaolan Wu, Yoko Shiroto, Sachie Kishitani, Yukihiro Ito, Kinya Toriyama, Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter Plant Cell Reports. ,vol. 28, pp. 21- 30 ,(2009) , 10.1007/S00299-008-0614-X
M. Okamoto, F. C. Peterson, A. Defries, S.-Y. Park, A. Endo, E. Nambara, B. F. Volkman, S. R. Cutler, Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance Proceedings of the National Academy of Sciences of the United States of America. ,vol. 110, pp. 12132- 12137 ,(2013) , 10.1073/PNAS.1305919110
Aiko Sato, Yuki Sato, Yoichiro Fukao, Masayuki Fujiwara, Taishi Umezawa, Kazuo Shinozaki, Takao Hibi, Mitsutaka Taniguchi, Hiroshi Miyake, Derek B. Goto, Nobuyuki Uozumi, Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase Biochemical Journal. ,vol. 424, pp. 439- 448 ,(2009) , 10.1042/BJ20091221