作者: Timothy J Triche Jr , Daniel J Weisenberger , David Van Den Berg , Peter W Laird , Kimberly D Siegmund
DOI: 10.1093/NAR/GKT090
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摘要: We propose a novel approach to background correction for Infinium HumanMethylation data account technical variation in fluorescence signal. Our capitalizes on new use the I design bead types measure non-specific colour channel opposite of their (Cy3/Cy5). This provides tens thousands features measuring instead much smaller number negative control probes platforms (n = 32 HumanMethylation27 and n 614 HumanMethylation450, respectively). compare performance our methods with existing approaches, using replicates both mixture samples biological samples, demonstrate that within- between-platform artefacts can be substantially reduced, concomitant improvement sensitivity, by proposed methods.