Effect of yeast culture on in vitro fermentation of a high-concentrate or high-fiber diet using equine fecal inoculum in a Daisy II incubator.

摘要: Two experiments were conducted to evaluate the use of a closed system, fermentation apparatus (Daisy II incubator) and determine effects yeast culture (YC) preparation (Saccharomyces cerevisiae) on in vitro microbial populations, diet digestion, patterns horses. In Exp. 1, 4 mature horses fed pelleted concentrate alfalfa cubes 50:50 (%, as-fed) ratio. Fecal samples taken from each horse form inoculum placed separate incubation vessels. Twenty nylon bags (10 with 0.25 g 10 0.50 total mixed diet) vessel, was carried out for 48 h DM, NDF, ADF digestibility. 2, fecal consuming either high-concentrate (HC) or high-fiber (HF) diet. Filter containing HC HF added vessels along their respective inoculums. Yeast 2 diet, whereas other served as controls. Vessels incubated 1 at 24 h. used ADF, OM digestibility, vessel fluid analyzed lactate, ammonia, VFA, concentrations. Results indicated that digestibility greater (P < 0.05), corresponding CV lower 0.05) 0.25- vs. 0.50-g sample size. YC tended = 0.10) decrease ammonia concentrations increased acetate production when compared control. There no pH, measured well The results did, however, show vivo DM estimates similar within Data 0.25-g size provides more accurate estimate less variation. Although had little, if any, effect Daisy incubator does provide valid tract horse. These data further evidence this process would be an effective practical means approximating diets varying forage ratios.