Preparation of a novel light-sensitive copolymer and its application in recycling aqueous two-phase systems.

摘要: Aqueous two-phase systems (ATPSs) are potential bioseparation techniques in industry. However, a key problem is that aqueous could not be effectively recycled to result high cost and environment pollution. Recently, how prepare recycling copolymers forming focused on the area. In this study, light-sensitive copolymer (P(NNC)) was synthesized by using N-isopropylacrylamide (NIPA), N-vinyl-2-pyrrolidone (NVP), chlorophyllin sodium copper salt (CHL) as monomers. The P(NNC) can form ATPSs with another novel pH-sensitive (P(ADB)) which co-worker our laboratory. Over 98% of recovered laser radiation at 488 nm. P(ADB) adjusting isoelectric point (pI) 4.1, recovery 97%. Bovine serum albumin (BSA) Tyr were partitioned P(NNC)-P(ADB) examine systems. It found partition coefficient BSA L-Tyr reach 4.1 0.12 systems, respectively.