Interactions between Auxin Transport and the Actin Cytoskeleton in Developmental Polarity of Fucus distichus Embryos in Response to Light and Gravity

摘要: Land plants orient their growth relative to light and gravity through complex mechanisms that require auxin redistribution. Embryos of brown algae use similar environmental stimuli developmental polarity. These studies the Fucus distichus examined whether transport are also required during polarization in early embryos already developed tissues. polarize with vector absence a cue. The auxin, indole-3-acetic acid (IAA), efflux inhibitors, such as naphthylphthalamic (NPA), reduced response vectors. Young rhizoids negatively phototropic, NPA inhibits rhizoid phototropism. effect IAA on photopolarization is maximal within 2.5 4.5 h after fertilization (AF). Over first 6 AF, relatively constant, suggesting developmentally controlled sensitivity determines narrow window which reduce polarization. Actin patches were formed hour AF began photolocalize 3 h, coinciding time action. Treatment polar localization actin but not patch formation. Latrunculin B prevented frame overlaps formation altered transport. Together, these results indicate role for orientation polarity suggest interactions between cytoskeleton F. embryos.