A High-Affinity Binding Protein for the Regulatory Subunit of cAMP-Dependent Protein Kinase II in the Centrosome of Human Cells

摘要: In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for regulatory subunits RII alpha beta of cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies several antibodies directed against show that these two isoforms are expressed. The major isoform is mostly cytosolic, whereas appears concentrated in Golgi-centrosomal area, as judged by immunofluorescence fractionation. Using a 32P-labelled overlay on Western blots, 350-kDa RII-binding (AKAP 350) was specifically identified centrosomes isolated from this line, Golgi fraction has previously been demonstrated to contain an 85-kDa 85). AKAP 350 highly insoluble can partially be extracted complex subunit. specific centrosomal pericentriolar material. potential significance subcellular distribution different proteins nonneuronal cells discussed.