A reagentless nitric oxide biosensor based on hemoglobin-DNA films
作者： Chunhai Fan , Genxi Li , Jianqin Zhu , Dexu Zhu
DOI: 10.1016/S0003-2670(00)01093-X
关键词:
摘要: Nitric oxide (NO) is an important bioregulatory molecule in vivo. A reagentless, hemoglobin (Hb) based nitric biosensor prepared, on the direct electrochemistry of Hb. Spectroscopic studies show that Hb maintains most its 3-dimensional structure film, and keeps reactivity with NO. The electrochemical reduction NO catalyzed employing modified electrode. Experimental results reveal peak current related to linearly proportional concentration. It can detect traces as low 2.9 μM. This also shown be simply constructed, biocompatible stable.
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