Examination of the binding behaviour of several proteins with the immobilized copper(II) complexes of o-, m- and p-xylylene bridged bis(1,4,7-triazacyclononane) macrocycles.
作者： Bim Graham , Leone Spiccia , Milton T.W. Hearn
DOI: 10.1016/J.CHROMA.2008.04.001
关键词:
摘要: Abstract Three new IMAC chelating systems, incorporating immobilised xylenyl-bridged bis(1,4,7-triaza-cyclonane) ligands, complexed with Cu2+ ions to form binuclear species, have been prepared. Their binding properties investigated three small globular proteins (hen egg white lysozyme, horse skeletal muscle myoglobin and heart cytochrome c). The effects of buffer pH, ionic strength composition on the behaviour these sorbents examined compared those found for corresponding immobilized mononuclear copper complex 1,4,7-triazacyclononane (tacn). Higher protein affinities were observed Cu2+-bis(tacn) Cu2+-tacn system, consistent copper(II) species undergoing enhanced coordinative interaction surface-exposed histidine residues proteins. Moreover, characteristics at higher strengths, such as 1 M NaCl, also reflect presence aromatic ring in bis(tacn) whereby hydrophobic π/π stacking interactions can occur
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sci-hub.se 参考文章(32)
Robert J. Todd, Robert D. Johnson, Frances H. Arnold, Multiple-site binding interactions in metal-affinity chromatography: I. Equilibrium binding of engineered histidine-containing cytochromes c Journal of Chromatography A. ,vol. 662, pp. 13- 26 ,(1994) , 10.1016/0021-9673(94)85291-X
Richard E. Dickerson, Tsunehiro Takano, David Eisenberg, Olga B. Kallai, Lalli Samson, Angela Cooper, E. Margoliash, Ferricytochrome c: I. GENERAL FEATURES OF THE HORSE AND BONITO PROTEINS AT 2.8 A RESOLUTION Journal of Biological Chemistry. ,vol. 246, pp. 1511- 1535 ,(1971) , 10.1016/S0021-9258(19)77002-1
Makonnen Belew, Tai-Tung Yip, Lennart Andersson, Jerker Porath, Interaction of proteins with immobilized Cu2+: Quantitation of adsorption capacity, adsorption isotherms and equilibrium constants by frontal analysis Journal of Chromatography A. ,vol. 403, pp. 197- 206 ,(1987) , 10.1016/S0021-9673(00)96353-2
T.William Hutchens, Tai-Tung Yip, Synthetic metal-binding protein surface domains for metal ion-dependent interaction chromatography. II. Immobilization of synthetic metal-binding peptides from metal ion transport proteins as model bioactive protein surface domains. Journal of Chromatography A. ,vol. 604, pp. 133- 141 ,(1992) , 10.1016/0021-9673(92)85538-5
Robert D. Johnson, Robert J. Todd, Frances H. Arnold, Multipoint binding in metal-affinity chromatography II. Effect of pH and imidazole on chromatographic retention of engineered histidine-containing cytochromes c Journal of Chromatography A. ,vol. 725, pp. 225- 235 ,(1996) , 10.1016/0021-9673(95)00992-2
Michele C. Smith, Thomas C. Furman, Charles Pidgeon, Immobilized iminodiacetic acid metal peptide complexes. Identification of chelating peptide purification handles for recombinant proteins Inorganic Chemistry. ,vol. 26, pp. 1965- 1969 ,(1987) , 10.1021/IC00259A030
B.H.J. Hofstee, Hydrophobic affinity chromatography of proteins Analytical Biochemistry. ,vol. 52, pp. 430- 448 ,(1973) , 10.1016/0003-2697(73)90046-8
Bim Graham, Peter Comba, Milton T. W. Hearn, Leone Spiccia, An examination of the binding behavior of histidine-containing peptides with immobilized metal complexes derived from the macrocyclic ligand, 1,4,7-triazacyclononane Journal of Biological Inorganic Chemistry. ,vol. 12, pp. 11- 21 ,(2006) , 10.1007/S00775-006-0160-3
Irving Langmuir, THE ADSORPTION OF GASES ON PLANE SURFACES OF GLASS, MICA AND PLATINUM. Journal of the American Chemical Society. ,vol. 40, pp. 1361- 1403 ,(1918) , 10.1021/JA02242A004
Frances H. Arnold, Metal-Affinity Separations: A New Dimension in Protein Processing Nature Biotechnology. ,vol. 9, pp. 151- 156 ,(1991) , 10.1038/NBT0291-151