In situ hybridization and immunohistochemistry of p53 tumor suppressor gene in human esophageal carcinoma.

摘要: We have studied expression of p53, a tumor suppressor gene, by using both immunohistochemistry and in situ hybridization 20 cases squamous cell carcinoma the esophagus. Immunohistochemical analysis was performed monoclonal antibody pAb1801. Immunoreactive p53 observed nuclei cells 17 cases. used 35S-labeled anti-sense single-stranded synthetic oligonucleotide probe ON102, which hybridized with DNA sequence near 5' end for hybridization. In all invasive studied, no significant accumulation signals cells. This result indicates that overexpression immunohistochemical staining is not due to an increase steady-state level mRNA frank six morphologically normal esophageal mucosa distant from carcinoma, basal parabasal mucosa. The these negative immunoreactivity except one case showing sporadic positivity. Accumulation foci dysplasia associated invasion three