Integrated use of phosphate-solubilizing Bacillus subtilis strain IA6 and zinc-solubilizing Bacillus sp. strain IA16: a promising approach for improving cotton growth.

摘要: Mineral nutrition of crop plants is one the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, availability phosphorus zinc critically less due to their fixation precipitation as complexes. Farmers use fertilizers fulfill requirements, but efficacy less, which increases production costs. Plant growth-promoting rhizobacteria (PGPR) can improve nutrients through solubilizing insoluble compounds soil. present study, a total 40 rhizobacterial isolates were isolated from cotton rhizosphere screened for improving growth solubilization zinc. Out these isolates, seven (IA2, IA3, IA6, IA7, IA8, IA13, IA14) efficiently solubilized rock phosphate while (IA10, IA16, IA20, IA23, IA24, IA28, IA30) more efficient oxide. liquid media, strain IA7 (2.75 μg/mL) highest amount concentration soluble was observed broth inoculated with IA20 (3.94 μg/mL). Seven phosphate-solubilizing zinc-solubilizing strains evaluated using jar trial seedlings, results quite promising. All treatments showed improvement parameters comparison control. Best shown combined application IA6 followed combination IA20. Based on trial, selected further characterized plant characters such siderophores production, HCN ammonia exopolysaccharides production. These identified 16S rRNA sequencing Bacillus subtilis (accession # MN005922), Paenibacillus polymyxa MN005923), sp. IA16 MN005924), aryabhattai MN005925). It hence concluded that integrated potential inoculants be promising approach under conditions.