XPACE4 is a localized pro-protein convertase required for mesoderm induction and the cleavage of specific TGFβ proteins in Xenopus development
作者： Bilge Birsoy , Linnea Berg , P Huw Williams , James C Smith , Christopher C Wylie
DOI: 10.1242/DEV.01599
关键词:
摘要: XPACE4 is a member of the subtilisin/kexin family pro-protein convertases. It cleaves many pro-proteins to release their active proteins, including members TGFβ signaling molecules. Studies in mouse suggest it may have important roles in regulating embryonic tissue specification. Here, we examine role Xenopus development and make three novel observations: first, is stored as maternal mRNA localized mitochondrial cloud vegetal hemisphere oocyte; second, required for endogenous mesoderm inducing activity vegetal cells before gastrulation; third, has substrate-specific activity, cleaving Xnr1, Xnr2, Xnr3 Vg1, but not Xnr5, Derriere or ActivinB pro-proteins. We conclude that XPACE4 plays an patterning by production of subset mature proteins specific sites.
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A.J. Roebroek, J.W. Creemers, I.G. Pauli, T. Bogaert, W.J. Van de Ven, Generation of structural and functional diversity in furin-like proteins in Drosophila melanogaster by alternative splicing of the Dfur1 gene. The EMBO Journal. ,vol. 12, pp. 1853- 1870 ,(1993) , 10.1002/J.1460-2075.1993.TB05834.X
L. Dale, G. Matthews, L. Tabe, A. Colman, Developmental expression of the protein product of Vg1, a localized maternal mRNA in the frog Xenopus laevis. The EMBO Journal. ,vol. 8, pp. 1057- 1065 ,(1989) , 10.1002/J.1460-2075.1989.TB03473.X
Richard E. MAINS, Carla A. BERARD, Jean-Bernard DENAULT, An ZHOU, Richard C. JOHNSON, Richard LEDUC, PACE4: a subtilisin-like endoprotease with unique properties Biochemical Journal. ,vol. 321, pp. 587- 593 ,(1997) , 10.1042/BJ3210587
Akihiko Tsuji, Kensuke Sakurai, Emi Kiyokage, Takahito Yamazaki, Sizuyo Koide, Kazunori Toida, Kazunori Ishimura, Yoshiko Matsuda, Secretory proprotein convertases PACE4 and PC6A are heparin-binding proteins which are localized in the extracellular matrix. Potential role of PACE4 in the activation of proproteins in the extracellular matrix. Biochimica et Biophysica Acta. ,vol. 1645, pp. 95- 104 ,(2003) , 10.1016/S1570-9639(02)00532-0
H. C. Huang, L. C. Murtaugh, P. D. Vize, M. Whitman, Identification, of a Potential regulator of early transcriptional responses to mesoderm inducers in the frog embryo The EMBO Journal. ,vol. 14, pp. 5965- 5973 ,(1995) , 10.1002/J.1460-2075.1995.TB00285.X
D.S. Kessler, D.A. Melton, Induction of dorsal mesoderm by soluble, mature Vg1 protein Development. ,vol. 121, pp. 2155- 2164 ,(1995) , 10.1242/DEV.121.7.2155
P.Huw Williams, Anja Hagemann, Marcos González-Gaitán, James C. Smith, Visualizing Long-Range Movement of the Morphogen Xnr2 in the Xenopus Embryo Current Biology. ,vol. 14, pp. 1916- 1923 ,(2004) , 10.1016/J.CUB.2004.10.020
Yanzhen Cui, François Jean, Gary Thomas, Jan L Christian, BMP-4 is proteolytically activated by furin and/or PC6 during vertebrate embryonic development The EMBO Journal. ,vol. 17, pp. 4735- 4743 ,(1998) , 10.1093/EMBOJ/17.16.4735
Séverine Beck, J. Ann Le Good, Marcela Guzman, Nadav Ben Haim, Karine Roy, Friedrich Beermann, Daniel B. Constam, Extraembryonic proteases regulate Nodal signalling during gastrulation. Nature Cell Biology. ,vol. 4, pp. 981- 985 ,(2002) , 10.1038/NCB890
D. A. Melton, Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes. Nature. ,vol. 328, pp. 80- 82 ,(1987) , 10.1038/328080A0