Photosynthesis Assessment in Microphytobenthos Using Conventional and Imaging Pulse Amplitude Modulation Fluorometry

作者: Sónia Vieira , Lourenço Ribeiro , Bruno Jesus , Paulo Cartaxana , Jorge Marques da Silva

DOI: 10.1111/J.1751-1097.2012.01224.X

关键词:

摘要: Imaging pulse amplitude modulated (Imaging-PAM) fluorometry is a breakthrough in the study of spatial heterogeneity photosynthetic assemblages. However, and conventional PAM uses different technology, making comparisons between these techniques doubtful. Thereby, processes were comparatively assessed using (Junior 101) Imaging-PAM on intertidal microphytobenthos (MPB; mud sand) cork oak leaves. Lower values α (initial slope rETR, relative electron transport rate) vs E (incident active radiation) curve), ETRmax (maximum ETR), Ek (light saturation parameter) Fv/Fm quantum efficiency photosystem II dark-adapted samples) obtained Imaging-PAM. The level discrepancy systems was dependent type sample, being more pronounced for MPB muddy sediments. This may be explained by differences depth integration fluorescence signal related to thickness layer light attenuation coefficients downwelling irradiance. An additional relevant parameter taxonomic composition MPB, as cyanobacteria present sandy sediments rendered results with red blue excitation fluorometers. These findings emphasize caution needed when interpreting chlorophyll data communities.

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