Transgenic avian species for making human and chimeric antibodies

摘要: The present invention provides methods for producing exogenous and chimeric antibodies in avians. One aspect of the is a method avians or avian cells lacking endogenous immunoglobulin light chain heavy loci, portions thereof, having at least portion one locus. obtaining an cell with deletion locus by introducing targeting construct comprising two regions sequences which are homologous to 5′ 3′ flanking region be deleted wild-type In addition, inserting gene loci into genome cell. A second generation transgenic species antibodies. host characterized by: (1) being incapable immunoglobulin; (2) constant thereof. Specific binding proteins xenogenic can produced viable immunization appropriate immunogen. Another isolation antibody-producing from that has been immunized antigen interest. immortalized production antibody culture. used cDNAs encoding chains reintroduced lines, including mammalian lines efficient monoclonal optionally mutated altered, example, such they encode higher avidity molecules, prior reintroduction lines.