Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science

摘要: This paper describes how the combination of genomics, genetic engineering, and 3D structural characterization has helped clarify redox regulatory networks in poplar with consequences not only system biology plants but also bacteria mammalian systems. Tree genomes are increasingly available a large number orphan genes coding for proteins, function which is still unknown. Modern techniques genome analysis coupled recombinant protein technology massive determination tree proteins should help elucidate many encoded by genes. X-ray crystallography NMR will be methods choice structure determination. In this review, we provide examples illustrating above-mentioned improved our understanding circuits poplar, first forest species sequenced. We showed that peroxiredoxins use either thioredoxin or glutaredoxin as electron donors to reduce hydrogen peroxide. That could reductant was unknown at time discovery even other biological organisms later confirmed notably observation two fused some resolution bacterial hybrid protein. Similarly, vitro enzymatic properties led plant methionine sulfoxide reductases can both thioredoxins glutaredoxins donors. Besides their disulfide reductase activity, it been demonstrated involved iron-sulfur center biogenesis assembly. The original made C1 then variety including human. Our work plants, so-called glutathione peroxidases true all non-selenocysteine-containing peroxidases. Finally, connections between systems have elucidated through study atypical thioredoxins. Altogether, these data illustrate engineering improves processes helps fuel trees species.