Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

摘要: Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce simple method enables proteomic imaging for scalable, integrated, high-dimensional phenotyping both animal tissues human clinical samples. This method, termed SWITCH, uniformly secures tissue architecture, native biomolecules, antigenicity across an entire system by synchronizing the preservation reaction. The heat- chemical-resistant nature resulting framework permits rounds (>20) relabeling. We have performed 22 labeling single with precise co-registration datasets. Furthermore, SWITCH synchronizes reactions to improve probe penetration depth uniformity staining. With combinatorial protein expression profiling cortex also interrogated geometric structure fiber pathways mouse brains. Such integrated information may accelerate our understanding biological systems at levels.