Clinical, serological, and molecular evidence of ehrlichiosis and anaplasmosis in dogs in Tunisia.

摘要: A seroepidemiological survey was conducted in five bioclimatic areas of Tunisia to determine the prevalence antibodies Ehrlichia canis and Anaplasma phagocytophilum antigens, surrogate markers agents canine monocytic ehrlichiosis (CME) granulocytic ehrlichiosis, respectively. Among 286 collected sera, 54.2% 25.2% were seropositive for E. A. phagocytophilum, respectively, by indirect immunofluorescence antibody (IFA) test. Clinical hematological tests done only 58 sick dogs from Tunis area. reverse line blot (RLB) hybridization then used identify isolated species infecting (n = 228). them, two infected phagocytophilum; ten sample demonstrated sp., which one dog showed a mixed infection with sp. RLB findings confirmed sequencing; BLAST search against GenBank revealed high similarity sequence PCR/RLB amplicons platys 16S rRNA partial sequence.