Chloroperoxidase-Mediated Halogenation of Selected Pharmaceutical Micropollutants

摘要: The oxidation of eight pharmaceutical micropollutants by chloroperoxidase derived from Caldaromyces fumago using hydrogen peroxide as an electron acceptor is reported. All the tested compounds, namely trazadone, sulfamethoxazole, naproxen, tetracycline, estradiol, ketoconazole, ketorolac, and diclofenac, were found to be substrates for chloroperoxidase. respective products identified electrospray ionization–mass spectrometry. contain at least one chloride atom in their structure after enzymatic oxidation. Degradability experiments indicated that most reaction are more biodegradable than corresponding unmodified compounds. enzyme was catalytically active effluent a water treatment facility, transforming with high rates conversions. immobilized chitosan macrospheres, which allowed catalyst recycled up three cycles simulated samples treated residual water. conversion first two cycles; however, third, 50% reduction capacity oxidize ketorolac observed. Additionally, immobilization improved performance over wider pH range, achieving 5, while free not this pH. Overall, results study suggest represents powerful potential terms its catalytic activity transformation micropollutants.