Detection of specific DNA sequences by flow cytometry

摘要: A method for detection of DNA sequences. In the method, chromatin (comprising protein and DNA) is contacted with a cross-linking agent to provide substantially rigid particle. The particle then subjected treatment cause separation individual strands into single stranded DNA. Preselected sequences are complementary polynucleotide probe specific sequence interest. marked fluorescent label thereby labelling target in particles. fluorescently tagged detected by subjecting suitable light source detecting emitted so as identify preselected sequence.