A combined vibrational sum frequency generation spectroscopy and atomic force microscopy study of sphingomyelin-cholesterol monolayers.

摘要: A combination of vibrational sum frequency generation spectroscopy and atomic force microscopy is used to study the changes in morphology conformational order monolayers prepared from three natural sphingomyelin (SM) mixtures as a function surface pressure cholesterol concentration. The most homogeneous SM gave with well-ordered acyl chains few gauche defects relatively small effects either increasing or addition. Heterogeneous mixture chain lengths significant fractions unsaturated had much larger contributions at low increasingly increased. They also showed substantial increases lipid after Overall, these results are consistent strong hydrogen bonding capacity leading over range pressures. for SMs relevant formation sphingolipid−cholesterol enriched domains cell membranes.