Stress-Related Proteins Are Specifically Expressed under Non-Stress Conditions in the Overwinter Buds of the Gentian Plant Gentiana triflora

摘要: The overwinter bud of the gentian plant, Gentiana triflora, is an organ that develops from basal tuber in late spring and becomes dormant freezing-tolerant for overwintering. Here, proteins specifically enriched buds were identified using two-dimensional polyacrylamide gel electrophoresis followed by amino acid sequencing. Enriched included those so far as stress- cold-inducible other plants, i.e., two isoforms ethylene-induced esterase, a dehydration-induced protein, glyoxalase I, thioredoxin peroxidase, ascorbate peroxidase membrane pore protein. Interestingly, stress-related had already accumulated under non-stress conditions early summer. Accumulation these was also examined mutant gentian, which developed but lacked cold tolerance died below 3°C. In this mutant, several including one putative enolase absent or greatly decreased. Thus, it appears are expressed to aquire before