Myosin Va is locally synthesized following nerve injury.

摘要: The presence of Myosin Va (an actin-based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within axons sciatic nerve demonstrated via immunocytochemistry. (M-Va) detected by using SDS-PAGE Western blot techniques with a polyclonal antibody specifically raised against M-Va globular tail domain. In addition, purification from rat prior to immunoblotting yielded standard band. Likewise, optical immunocytochemical procedures revealed M-Va, particularly cortical axoplasmic territory, but also Schwann cell soma. above experiments were carried out both on intact as well severed nerves similar results. proximal stumps (from 0 72 h after injury) labelled vivo 35 S-methionine. autoradiography immunoabsorbed radiolabelled homogenized tissue showed significant increment radioactive intensity heavy chain band through time. Moreover, transcripts coding for time RT-PCR injury compared nerves. This data suggest that is up-regulated timedependent manner. latter suggests possible involvement regeneration processes. Cell Motil. Cytoskeleton 51:169‐176, 2002. © 2002 Wiley-Liss, Inc.