Flow cytometry as a method for assaying the biological activity of phytotoxins
作者： Deborah L. Berglund , Scott Strobel , Fumio Sugawara , Gary A. Strobel
DOI: 10.1016/0168-9452(88)90033-7
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摘要: Abstract A flow cytometric method has been developed for the qualitative and quantitative evaluation of biological activities phytotoxins from plant pathogenic fungi. The utilized fresh wheat ( Triticum aestivum L.) leaf protoplast preparations treated with purified phytotoxins, triticone A-B D. Subsequently, protoplasts were exposed to fluorescein diacetate, analyzed by cytometry. Information acquired included fluorescence owing esterase activity on chlorophyll autofluorescence. Results indicate that a rapid dose-dependent toxic effect but D no effect. This can also yield information mechanism action are relatively unstable or available only in small quantities.
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