LncRNA LINC01116 Promotes the Development of Colorectal Cancer by Targeting miR-9-5p/STMN1.
作者： Chongyao Bi , Hongshuai Cui , Haijing Fan , Lai Li
DOI: 10.2147/OTT.S253532
关键词:
摘要: Purpose The aim was to research the role of LINC01116 in prognosis colorectal cancer (CRC) patients and development cells. Methods In total 62 patient tissues human CRC cell lines (OUMS23, SW116, SW480 LOVO) were obtained for this study. SiLINC01116, miR-9-5p mimic, LINC01116, oe-STMN1 their controls transfected. qRT-PCR method Western blot used detect levels STMN1 CCK8 assay flow cytometry processed proliferation apoptosis, respectively. Transwell undertaken verify invasion migration. Luciferase pull down confirm binding relationship among STMN1. Immunohistochemistry also detected expression Kaplan-Meier survival curve analyze rate. Pearson correlation analysis evaluate regulatory between tissues. Results expressed higher Patients with had worse prognosis. Knockdown suppressed cell. negatively regulated miR-9-5p, while MiR-9-5p related mimic could rescue effect inhibit migration invasion, improve apoptosis Oe-STMN1 on cancer. Conclusion promoted via modulating miR-9-5p/STMN1 axis.
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